Simple Method for Constructing Internal Standards for Competitive PCR
نویسندگان
چکیده
منابع مشابه
designing novel and simple competitive internal amplification control for reliable pcr diagnosis of herpes simplex virus
background pcr is a molecular technique for herpes simplex virus (hsv) detection that can cause life-threatening infections such as encephalitis and keratitis. however, the main issues, false-negative results causing by pcr inhibitors, of this technique that reduce pcr efficiency. to overcome this problem, a competitive internal amplification control (iac) was constructed for conventional pcr u...
متن کاملPreparing polyA-containing RNA internal standards for multiplex competitive RT-PCR.
PCR-based strategies such as competitive RT-PCR or semiquantitative RTPCR are commonly used for quantitating low-abundance mRNA transcripts (4,7–9). Competitive RT-PCR relies on amplifying the same target with a known amount of a competitor/reference sequence and comparing the relative amounts of the two PCR products. To improve the sensitivity and accuracy of these methods, often expensive and...
متن کاملDesigning Novel and Simple Competitive Internal Amplification Control for Reliable PCR Diagnosis of Herpes Simplex Virus
BACKGROUND PCR is a molecular technique for herpes simplex virus (HSV) detection that can cause life-threatening infections such as encephalitis and keratitis. However, the main issues, false-negative results causing by PCR inhibitors, of this technique that reduce PCR efficiency. To overcome this problem, a competitive internal amplification control (IAC) was constructed for conventional PCR u...
متن کاملSerial competitive RT-PCR using multiple standards.
PCR is widely used for the amplification of DNA and reverse-transcribed RNA. In recent years, real-time PCR has been introduced as a new technique for mRNA quantitation. However, because of the high cost of real-time PCR equipment and supplies, especially using custom-made probes, quantitative RT-PCR is a valuable alternative method. Several quantitative PCR protocols describe the use of a sing...
متن کاملQuantitative RT-PCR using a PCR-generated competitive internal standard.
One approach to quantitate RNA is the use of a known number of competitive internal standard molecules that are reverse-transcribed and subsequently co-amplified under the same reaction conditions as the target sequence (3). This technique, called competitive reverse transcription polymerase chain reaction (cRT-PCR) has been applied to monitor the level of hepatitis C virus (HCV) RNA in patient...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96213bm07